tools for Be the first to review our Ponceau S Staining Solution and receive a gift card or discount. If you know of a relevant reference for Ponceau S Staining Solution, please let us know. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). The technical data provided above is for guidance only. Get resources and offers direct to your inbox. ab270042 has not yet been referenced specifically in any publications. This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our. apply to Products provided by CST, its affiliates or its distributors. review today?! Azide-containing monosaccharide building block; used as glycoprotein, glycolipid and glycoRNA probe, Amine reactive biotinylation reagent, cell permeable. Expedeon product code 5999-1L is the same as the1000 mL size of this product. Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Get the latest news, product updates, and promotions: Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.. Best staining for tracking protein transfer in western blot. Fluorescent Probes and Dyes Product Guide, Citations for Ponceau S Staining Solution, GMP & Ancillary Material Grade Small Molecules, Certificate of Analysis / Product Datasheet, Submit a Review prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, Simply enter the mass of reagent and the target concentration and the calculator will determine the Customer shall not use any Product for any diagnostic Mar 23 2019 [PMID: 30914243] (WB), Review with no image -- $10/7/6/$10 CAD/70 Yuan/1110 Yen, Review with an image -- $25/18/15/$25 CAD/150 Yuan/2500 Yen. There are currently no reviews for this product. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your Stain used to detect protein bands after Western blotting, 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). We use Ponceau S (Cat. Support products are. All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce background staining on the blotting membrane. To get a clear background, wash the membrane quickly in PBS after staining2. Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). For batch specific data refer to the Certificate of Analysis. requires a separate license from CST. The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. There are no specific FAQs related to this product. Store at room temperature. As little as 250 ng protein can be rapidly visualized with Ponceau S stain. The intensity of staining corresponds to the amount of protein transferred. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Your It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Do not dilute or aliquot. industry. Lanes 1 to 8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2 g BSA, lane 5 1 g BSA, lane 6 500 ng BSA, lane 7 250 ng BSA. review Ponceau S Staining Solution and earn rewards! *Please note that Tocris will only send literature to established scientific business / institute addresses. Wash membrane in ddH2O until distinct reddish-pink protein bands are visible (1-5 min). Following protein electrotransfer, rinse membrane briefly in ddH2O to remove any detergent that may inhibit staining. are provided for Customer as the end-user and solely for research and development uses. Ponceau S waste: Ponceau S staining for total protein normalization Anal. Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). Take a look at our BETA site and see what weve done so far. Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. CST Product Terms of Sale and any applicable The technical data provided above is for guidance only. Guaranteed product quality, expert customer support. Tocris products are intended for laboratory research use only, unless stated otherwise. 1998-2022 Abcam plc. No product specific FAQs exist for this product, however you may. $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Your browser does not have JavaScript enabled and some parts of this website will not work without it. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. We offer a 100% guarantee on all our products. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Buffers, Chelators and Reagents Staining Kits and Reagents, View Full Technical Details for Ponceau S Staining Solution. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed For batch specific data refer to the Certificate of Analysis. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Tocris offers the following scientific literature in this area to showcase our products. or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Alternatively, a photograph of the stained membrane can also be taken. by the FDA or other regulatory foreign or domestic entity, for any purpose. All Rights Reserved. life science research with over 30 years' experience in the 2022 Novus Biologicals, All Rights Reserved. copyright notices or markings, (d) use the Products solely in accordance with documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. Tocris is a Bio-Techne brand. Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. Note: Mouseover a species abbreviation on the product page to display the fullname. Be the first to Citations are publications that use Tocris products. Calculate the mass, volume, or concentration required for a solution. Do you know of a great paper that uses Ponceau S Staining Solution from Tocris? To download a Certificate of Analysis, please select a batch below. any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any This ponceau S solution was used to stain total protein that had been applied to PVDF membrane using during a slot blot experiment. View all citations for the Staining Kits and Reagents platform. Calculate the dilution required to prepare a stock solution. We invite you to request* your copy today! You can now submit reviews for your favorite Tocris products. If desired, mark proteins with waterproof ink or a pencil. Keywords: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience. Tocris products are intended for laboratory research use only, unless stated otherwise. Visit our, Salinovich, O. and Montelaro, R.C. 1. Which Brands are Currently Available on biotechne.com? Products sold or licensed by CST Why not submit a This enables one to incubate the different membrane sections with various primary antibodies. TBS/BSA). Proceed with blocking step of Western blot procedure. The reviewer received a reward from Abcams Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. 5. 4. Store at Room Temperature. addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized Publishing research using ab270042? Wash membrane in 1X TBS-T several times for 5 min at room temperature until protein bands are no longer visible. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Any use of Product for diagnostic, rest. Read our general, There are no specific blogs for Ponceau S Staining Solution, but you can. Toll-free 800 343 7475 Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt. This enables one to incubate the different membrane sections with various primary antibodies. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. View all citations for the Staining Kits and Reagents platform. Please let us know. Shipped at Room Temperature. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, Explore the power of knock-out cell lines for your research. 2. Bacterial peptidoglycan derivative; suitable for 'click'-conjugation to fluorescent dyes. vial. For batch specific data refer to the Certificate of Analysis. All rights reserved. review will help other researchers This product is for research use only and is not approved for use in humans or in clinical diagnosis. Ponceau red stain is not stable and will disappear if you destain longer.3. Get the latest news, product updates, and promotions: Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). Copyright 2022 Bio-Techne. This product is stable for 12 months. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). This product is manufactured by Expedeon, an Abcam company. It was previously called Ponceau S Solution - 1L. structure or technology of the Products, or use the Products for the purpose of developing any products or services that would Our guide highlights the use of Fluorescent Probes and Dyes in research and provides background information on various imaging techniques. decide on the best products for their research. USA, TEL 612 379 2956 1. Incubate membrane in Ponceau S Staining Solution for 5-10 minutes at room temperature. (1986). No. There are no reviews for Ponceau S Staining Solution (NB5225). Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE services used by Customer in connection with the Products. NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact partnerships@abcam.com. Constituents: 0.1% Ponceau S, 0.96% Acetic acid. References are publications that support the biological activity of the product. Any Customer's terms and conditions that are in There are no To get a clear background, wash the membrane quickly in PBS after staining2. Following staining, membranes can be de-stained with any buffer of pH >7 or by transfer of the membrane into blocking buffer (e.g. Minneapolis, MN 55413 This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. By continuing to use this website you acknowledge this and agree to our cookie policy. Changing to another country might result in loss of shopping cart. Please contact us to place your order, or try again later. Ponceau S Staining Solution Support Products, View other related Ponceau S Staining Solution Support Products, Sander H, Wallace S, Plouse R et al. Currently there are no citations for Ponceau S Staining Solution. Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs. We have publications tested in 1 application: WB. Average Rating: 4.3 (Based on 3 Reviews. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or 30 ug of protein was loaded per well and image was taken using a LiCor Odyssey imager The staining worked but the signal was not as strong as other Ponceau S stains I have used before. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. No. Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes. the vial label and CoA (available online). & Earn an Amazon Gift Card, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution), TSA Reagents for Enhancing IHC, ICC & FISH Signals. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services. reviews that match your criteria. 3. Would you like to visit your country specific website? The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Products in stock will be shipped in 1-3 business days unless specified. Purchase these through your usual distributor. The technical data provided above is for guidance only. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Agonists, activators, antagonists and inhibitors, Anti-CYP2C9 antibody [EPR7340] (ab150364). ACD branded products will be available on biotechne.com in the near future. Bio-Techne uses cookies to provide you with a great website experience. Copyright 2022 R&D Systems, Inc. All Rights Reserved. FAX 612 656 4400. 6. ), $50/35/30/$50CAN/300 Yuan/5000 Yen for first to review with an image, $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections. Ponceau red stain is not stable and will disappear if you destain longer.3. *When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online). Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available Tocris Bioscience is the leading supplier of novel and exclusive representative of CST, are rejected and are of no force or effect. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. We use Ponceau S (Cat. Tocris products are intended for laboratory research use only, unless stated otherwise. Please note: All products are "FOR RESEARCH USE ONLY. 1. *When preparing stock solutions always use the batch-specific molecular weight of the product found on Please let us know so that we can cite the reference in this datasheet. Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. Biochem. ProteinSimple branded instruments are available to quote.